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KMID : 0545120090190050525
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 5 p.525 ~ p.529
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Detection of Cytolethal Distending Toxin and Other Virulence Characteristics of Enteropathogenic Escherichia coli Isolates from Diarrheal Patients in Republic of Korea
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Kim Jong-Hyun
Kim Jong-Chul
Choo Yun-Ae
Jang Hyun-Chul
Choi Yeon-Hwa
Chung Jae-Keun
Cho Seung-Hak
Park Mi-Seon
Lee Bok-Kwon
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Abstract
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Cytolethal distending toxins (CDTs) represent an emerging family of newly described bacterial products that are produced by a number of pathogens. The genes encoding these toxins have been identified as a cluster of three adjacent genes, cdtA, cdtB, and cdtC, plus 5 cdt genetic variants, designated as cdt-I, cdt-II, cdt-III, cdt-IV, and cdt- V, have been identified to date. In this study, a general multiplex PCR system designed to detect Escherichia coli cdts was applied to investigate the presence of cdt genes among isolates. As a result, among 366 E. coli strains, 2.7% were found to carry the cdtB gene. In addition, the use of type-specific primers revealed the presence of cdt-I, cdtIV, and cdt-V types of the cdt gene, yet no cdt-II or cdt- III strains. The presence of other virulence genes (stx1, stx2, eae, bfp, espA, espB, and espD) was also investigated using a PCR assay. Among the 10 cdtB gene-positive strains, 8 were identified as CDT-producing typical enteropathogenic E. coli (EPEC) strains (eae+, bfp+), whereas 2 were identified as CDT-producing atypical EPEC strains (eae+, bfp-). When comparing the cytotoxic activity of the CDT-producing typical and atypical EPEC strains, the CDT-producing atypical EPEC strains appeared to be less toxic than the CDT-producing typical EPEC strains.
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KEYWORD
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Cytolethal distending toxin, atypical EPEC, typical EPEC, bundle-forming pilus
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